Label retaining assay
Tīmeklis2016. gada 8. jūn. · It was concluded that label-retaining cells in glioblastoma spheres manifest clear stem cell features and that the label-retaining assay may be utilized …
Label retaining assay
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Tīmeklis2008. gada 1. jūn. · Label retention assays remain the state‐of‐the‐art approach to identify the location of intraorgan epithelial stem cell niches, in situ and in vivo. They are commonly used in organs with rapid cell turnover but have not been applied to the liver, where cell turnover is very slow. We used a sublethal dose of acetaminophen … Tīmeklis2024. gada 28. jūl. · Single prostate stem cells can generate stem and progenitor cells to form prostaspheres in 3D culture. Using a prostasphere-based label retention …
Tīmeklis2014. gada 19. sept. · The chase period for the label retaining assay was 8 weeks, and at this time point the mice were anesthetized via an intraperitoneal injection with … Tīmeklis2013. gada 1. janv. · Importantly, when cells were marked with H2B-GFP then chased for 20 months, at least two distinct label-retaining cell ... In this assay, aged H2B-GFP+ satellite cells showed an ...
TīmeklisAbstract. Label retention assays remain the state-of-the-art approach to identify the location of intraorgan epithelial stem cell niches, in situ and in vivo. They are … Tīmeklis2016. gada 8. jūn. · Label-retaining cells, which are characterized by dormancy or slow cycling, may be identified in a number of human normal and cancer tissues, and …
TīmeklisAbstract. Hematopoietic stem cells (HSCs) are thought to divide infrequently based on their resistance to cytotoxic injury targeted at rapidly cycling cells and have been presumed to retain labels such as the thymidine analog 5-bromodeoxyuridine (BrdU). However, BrdU retention is neither a sensitive nor specific marker for HSCs.
TīmeklisLabel retaining cell (LRC) assay remains the well-known method to identify possible somatic stem/progenitor cells and their location both in situ and in vivo. Methods: … choose well careTīmeklis2009. gada 3. okt. · Label retention cell assay is a viable method used to locate stem cells and to identify them as slow dividing, quiescent or segregating chromosomes asymmetrically [10, 27, 33]. ASCs can be labeled by DNA synthesis precursor analogues such as 3 H-TdR or bromodeoxyuridine (BrdU) during development or … great affect or great effectTīmeklisA label retention assay—similar to that performed by Cotsarelis et al. (1990)—was then used to identify slow-cycling cells, which were assumed to be stem cells and … choose wedding dressTīmeklis2013. gada 1. maijs · Label-retaining cells (black nuclei) represent putative stem cells and are located in the canals of Hering as keratin-expressing cells. ... Landis CS, Swenson ES, Barendswaard E, Theise ND. The hepatic stem cell niche: identification by label-retaining cell assay. Hepatology. 2008;47(6):1994–2002. View this article via: … great affiliate products to promoteTīmeklis2004. gada 1. sept. · Epidermal label-retaining cells (LRC) can be identified by giving neonatal mice repeated injections of 3 H-thymidine or 5-bromo-2′-deoxyuridine and … great a email accountTīmeklis2024. gada 22. maijs · Label retention assays exploit the dilutional nature of cell divisions to estimate how many mitoses a cell experienced since being marked with a fluorescent label. Labels can be in the form of chemical dyes [] or experimentally expressed fluorescent molecules [[88-90]]. The salient requirement is that it cannot … great adwords adsTīmeklis2012. gada 19. marts · Slow-dividing somatic stem cells have been identified for many years by using variations of the label retention assay (Figure 1), which is based on … great aether