WebFITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining as described in the FITC Annexin V Staining Protocol. Investigators should note that FITC Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane Web1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5.

Milli-Mark® Anti-FcεRI Antibody, γ subunit-FITC MSDS

WebDo you offer FITC labeled primers? IDT offers a 6-FAM fluorophore modification instead of the traditional FITC fluorophore modification. FITC and FAM are both derivatives of … WebThere are several reagents that may be used with FITC and/or TRITC derivatives to prevent fading, including n-propyl gallate at 0.1-0.25M dissolved in glycerol. 2. For FITC … chinese association of automation

Milli-Mark® Anti-FcεRI Antibody, γ subunit-FITC MSDS

WebFluorochrome conjugated antibodies for flow cytometry Section 1. Identification Human CD3/RPE // 0.2-100mL FR868 // MultiMix Dual-Colour Reagent, Anti-Human CD4/FITC + Anti-Human CD8/RPE // WebThe FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in situ monitoring of caspase activity by fluorescence detection. The suggested concentration for use in anti-Fas-treated Jurkat cell culture is 10μM. Features and Benefits. A fast and simple protocol; just add FITC-VAD-FMK, incubate, wash and view fluorescence. WebSafety Data Sheet for Fluorescein sodium (C.I. 45350) 103887 Material Safety Data Sheet or SDS for Fluorescein sodium (C.I. 45350) 103887 from Merck for download or viewing in the browser. Catalog Number 103887 Product Name Fluorescein sodium (C.I. 45350) chinese aster facts