Commonly misidentified cell lines
WebJan 24, 2024 · The pervasiveness of cross-contaminated and misidentified cell lines is a decades-long issue; in 1967, cell lines thought to be derived from various tissues were shown to be HeLa cells, a human cervical adenocarcinoma cell line. 1 However, studies involving these misidentified cell lines continued to feature in hundreds of citations … WebThe recommended procedure for anyone contemplating the use of cell lines is as follows: Check that the cell line that you intend to use is not listed in the above database. Ensure that the cell line is obtained from a properly authenticated source (and that may not be the originator), preferably from one of the recognized cell banks.
Commonly misidentified cell lines
Did you know?
WebContaminated and misidentified cell lines commonly use in cancer research Cell line authentication is important for credibility concern and scientific reproducibility. Authenticated cancer cell lines retain the properties of the cancers of origin and serve valuable resources for medical research. WebNov 27, 2015 · The MDA-MB-435 cell line used in Supplementary Fig. 2e–g is found on the list of commonly misidentified cell lines that is maintained by ICLAC and NCBI BioSample, where it is listed as a...
WebProblematic cell line: Misidentified. Originally thought to be a hepatocellular carcinoma cell line but shown to be from an hepatoblastoma (PubMed=19751877). Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: ENCODE project common cell types; tier 1. Part of: JFCR45 cancer cell line panel. Part of: MD Anderson Cell Lines Project. WebApr 10, 2024 · MSI is rare (<1%) in many other tumour types, such as kidney, melanoma and prostate cancers 17 and most (4 out of 5 tested) MSI cell lines from these tissues were not dependent on WRN (Extended...
WebApr 17, 2024 · Indeed, worldwide, between 14 and 46% of the most commonly used cell lines are believed to be incorrectly designated [7 ... From January 2010 to January 2016, using the misidentified cell line HBL-100, we were able to find only two articles that referred to this cell line in the title or abstract. Both were submitted in 2009, which was … WebHEK293T cells (ATCC® CRL‐3216™, RRID: CVCL_0063) (the cell line is not listed as a commonly misidentified cell line by the International Cell Line Authentication Committee) were cultured… No further authentication was performed in the laboratory. A maximum of 6 cell passages was used. All data are presented as mean ± SE.
WebMake sure your cells have not been misidentified and reclassified. Cell lines are among the most used in vitro models representing normal and disease tissue in basic research …
WebJul 22, 2024 · Cell lines were free of mycoplasma, authenticated on the basis of morphology and growth properties and confirmed by PCR to be of the correct species. The cell lines used were not listed as... technician x rayWebNov 20, 2024 · Where do all these Misidentified Cell Lines Come From? In some cases, the cell type or tissue origin of cells grown are incorrect from the outset. Researchers … technicien at mpWebATCC offers scientists a range of high-quality cell authentication testing services backed by nearly a century of experience in biomaterial management and authentication standards. … technicien easyfoneWebSeveral tests for authenticating cell lines have been established as means of combatting the issues of misidentification and cross contamination. For intraspecies contamination … technician weavileWebPolicy information about cell lines Cell line source(s) HEK293T cells were obtained from ATCC (CRL-11268). Expi293F cells were purchased from ThermoFisher Corporation. … technicien a1WebSep 13, 2024 · In this review, we highlight the common pitfalls when working with antibodies, common practices for validating antibodies, and levels of commercial antibody validation for seven vendors. ... First step (and simplest): check your cell line(s) against the list of known cross-contaminated or misidentified cell lines available on the NCBI … technic icontechnicien de recherche et formation cn