WebOct 9, 2024 · The cycloheximide (CHX) ... Gefitinib, afatinib, AZD9291, MG132, Baf A1, Amiloride HCl, and dyngo-4a were purchased from Selleck (Houston, TX, USA). Laemmli sample buffer (2×) and an ECL ... WebApr 8, 2024 · A DLD1 cells were treated with MG132 (20 μM) for 5 h, lysed and subjected to immunoprecipitation with anti-PRPF19 antibody or IgG using silver staining. The red arrow represents the detected bands. ... (Ctrl/PRPF19) were treated with CHX, and lysed for WB analysis at the specific time points. The intensity of MYL9 expression for each time ...
The effect of MG132, a proteasome inhibitor on HeLa cells in ... - PubMed
AGR2 protein is highly expressed in various human cancers including lung cancer [12], which is associated with poor patient survival [4] and also confirmed in our analysis (Fig. 1a). Consistent with the report [15], AGR2 was preferentially expressed in non-small cell lung cancer (NSCLC) H460 and A549 cells (Fig. 1b). … See more Hormones and several transcriptional factors, including androgen receptor (AR), FoxA1, and FoxA2, are implicated in positive regulation of AGR2 expression [12]. We found that … See more To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was included in the study below. The AGR2 abundance was reduced by CHX, and this … See more As AGR2 was polyubiquitinated in response to MG132, ubiquitin mutant vectors containing K48, K63, K48R, and K63R (K-to-R … See more WebThe CHX and MG132 stocks were prepared in water and DMSO, respectively, and all control experiments contained an equal amount of solvent. Immunoblotting analysis Total protein extracts were prepared as described previously, 21 separated by SDS-PAGE, and transferred to nitrocellulose membranes (Bio-Rad). try to watch 3 minutes without laughing
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WebCycloheximide (CHX) (Sigma Aldrich) is stored as a 500 μg/mL stock solution, solved in dimethylsulfoxide (DMSO) at − 20°C in small aliquots. Proteasome inhibitor MG132 (Enzo Lifesciences) is prepared as a 10 m M stock solution in … WebMay 26, 2024 · Enhancer of zeste homolog 2 (EZH2) is the catalytic subunit of polycomb repressive complex 2 (PRC2), which silences transcription through trimethylation of histone H3 lysine 27 (H3K27me3) and has... WebFeb 20, 2024 · Materials and methods 2.1. Chemical compounds and cell lines Bufotalin (BT) was purchased from MedChem Express (New Jersey, USA). Ferrostatin-1, Deferoxamine, MG132, and Cycloheximide were purchased from MedChem Express (New Jersey, USA). C11-BODIPY 581/591 were purchased from ThermoFisher Scientific (MA, … try to watch 意味